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I brush two times a day and I haven't had an issue. Only caveat is that I brush very thoroughly. There was this brief period of time where I was swamped with schoolwork and I brushed once per day for a handful of days in a row (I was up until the wee hours of the morning toiling away on my work and I wasn't motivated to stay up five minutes longer to brush my teeth)...but then I got cavities so I'm never doing that again.
